TBS (Tris Buffered Saline)

1. Tris base................................6.06g

2. Sodium Chloride.................8.76g

3. 1000ml dH20

4. 2ml Brij 35

Dissolve 6.06g of Tris base in 90ml of dH2O

Adjust pH to 7.6 with concentrated HCl and bring total volume up to 100ml with dH2O.

Dissolve 8.76g of sodium chloride in 100ml of dH2O

Mix 100ml of tris base and 100ml of sodium chloride with 800ml dH2O to yield 1L of TBS (0.05M Tris, 0.15M NaCl, pH 7.6)

Add 2ml of Brij 35 (detergent used to lower surface tension of water.