1. Harvest rat tail tendon sheaths and store in 70% ethanol.
2. Weigh out 5g of tendons and place in an Erlenmyer flask containing 100ml of 1% glacial acetic acid.
3. Place on a stir plate in the cold storage room and stir at medium speed until almost clear. Make 10, 10ml Aliquots and store at 4°C or freeze at -20°C for long term storage.
4. Slides are coated with one drop of rat tail collagen using a transfer pipette just prior to use in the water bath. The drop should be spread evenly across the surface of the slide.