METHACARN PROTOCOL:
FIXATION AND PROCESSING

Tissue can be fixed in methacarn in as little as four hours. Specimens can be stored in fixative for several weeks without appearent harm to tissue morphology or antigenicity.

METHACARN FIXATIVE:

60% ABSOLUTE METHANOL (1800ml)

30% CHLOROFROM (900ml)

10% GLACIAL ACETIC ACID (300ml)

Tissues are rinsed in one change of 70% ethanol prior to processing with the VIP tissue processor. Use program # 6 for overnight processing or program # 2 for weekend processing. Both programs begin with 70% ethanol and end in molten paraffin.