Tissue can be fixed in methacarn in as little as four hours. Specimens can be stored in fixative for several weeks without appearent harm to tissue morphology or antigenicity.
60% ABSOLUTE METHANOL (1800ml)
30% CHLOROFROM (900ml)
10% GLACIAL ACETIC ACID (300ml)
Tissues are rinsed in one change of 70% ethanol prior to processing with the VIP tissue processor. Use program # 6 for overnight processing or program # 2 for weekend processing. Both programs begin with 70% ethanol and end in molten paraffin.