Harris Hematoxylin (Stock)

1. Hematoxylin.............................................5g

2. Absolute ethanol...................................50ml

3. Ammonium aluminum sulfate.................100g

4. Distilled water...................................1000ml

5. Sodium iodate.....................................0.37g

Dissolve the hematoxylin in the alcohol using a stir bar (it may be necessary to add 20ml of the water to completely dissolve the hematoxylin). Completely dissolve the ammonium aluminum sulfate in water with heat. Remove from heat and mix the two solutions. Bring to a boil as rapidly as possible. Remove from heat and add the sodium iodate. Reheat to boiling and boil for 2 - 3 min. Remove from heat and plunge into an ice bath. Allow to cool and filter using 541 filter paper. Store in brown bottle at room temp (solution in light sensitive). Should be stable for 2 months. To test potency, place a few drops on a piece of filter paper. The paper should be a maroon color with a purple edge at the top of the paper. If you are unable to distinguish the two colors, discard the solution and make fresh.

 

Working Harris Hematoxylin:

A. 200ml of stock Harris hematoxylin (filtered)

B. Add 8ml of glacial acetic acid (4ml of acid/100ml of hematoxylin)