1. 1000ml flask with 150ml of PBS
2. Dissolve 42.8gm of sucrose
3. Add 0.7gm of magnesium chloride crystals
Pour mixture into a graduated cylinder and add PBS to bring the vol. up to 250ml, then add 250ml of glycerol and stir well. Store the medium at -10 to -20oC. The slides will remain stable for three months in this medium. Before proceding with immunostaing rinse medium off with two changes of PBS. The storage medium itself is stable for up to 1 year.
Revised - July 24, 1992