FIXATION PROTOCOL FOR CELL CULTURE SLIDES

 1. Drain excess media from the slide cell culture wells.

2. Remove the plastic well cover (leave the gasket on the slide).

3. Place directly in 10% neutral buffered formalin and fix for 30 min. at room temp.

4. Rinse in distilled water (3 changes), 30 sec. each.

5. Place one set in 70% ethanol and store at room temp. Rinse the other set in PBS pH 7.4 and then place in frozen specimen storage medium (store at -20 C) until ready for IHC procedure.