1. Drain excess media from the slide cell culture wells.
2. Remove the plastic well cover (leave the gasket on the slide).
3. Place directly in 10% neutral buffered formalin and fix for 30 min. at room temp.
4. Rinse in distilled water (3 changes), 30 sec. each.
5. Place one set in 70% ethanol and store at room temp. Rinse the other set in PBS pH 7.4 and then place in frozen specimen storage medium (store at -20° C) until ready for IHC procedure.