EOSIN Y

Stock Eosin Y (1000ml)

1. Eosin Y (Fisher Cat. # E-511)........10g

2. 95% ethanol..............…………..1000ml

Use a 250ml Erlenmyer flask with a stir bar to dissolve the eosin in the 95% ethanol. Filter using a #541 or similar filter. Place in a brown bottle and store at room temp. The stock solution should be stable for several months.

Working Eosin Y (200ml):

A. 180ml of stock Eosin Y (filtered)

B. 20ml of Methanol

C. 1ml of glacial acetic acid

The working solution should be stable for 2 - 3 weeks depending on how many slides are stained.