Alum Carmine Stain (0.4%)

Carmine (Fisher Scientific, # C579-25).....…………..4g

Aluminum potassium sulfate .........…………………..10g

Distilled water.................…………………………...1000ml

Dissolve carmine and alum potassium sulfate in 800ml of dH2O and bring to a boil. Filter (#1 Whatman) while warm and adjust final volume to 1000ml with dH2O. Add 1 crystal of thymol as a preservative. Store the solution in a brown glass bottle at room temp. The stain should be stable for 2 months.