ER & PR

 
Click on any image to enlarge

ER 1-D5 expression in normal rat mammary duct. Detection with Biogenex Super Sensitive HRP kit. Positive nuclei (arrows), negative nuclei (arrowheads). The high background stain resulting from this kit makes image analysis difficult if not impossible.
 
 
ER 1-D5 (DakoCytomation) expression in normal rat mammary gland. Detection with DakoCytomation Envision+ HRP kit. Positive nuclei (arrows), negative nuclei (arrowheads).  This kit uses a dextran coated polymer for detection and delivers a very crisp, clean immuno stain. The absence of biotin and streptavidin in the system reduces background to a minimum.
 

ER (1-D5) expression in papillary adenocarcinoma. The first layer of epithelium, adjacent to the stromal tissue is negative for ER expression. A similar pattern of expression is also seen with PR.

 

 
ER (1-D5)expression in cribriform adenocarcinoma. Innermost epithelial cells comprising secondary lumina are mostly negative. Mitotic figures are indicated by arrowheads.
   
PR (PR88 Biogenex) expression in cribriform adenocarcinoma.
     

 

 

 

 

 

 

 

Colorado State University CHOICE BreastWatch